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VeilleNanos - Risks associated with silica nanoparticles

Risks associated with silica nanoparticles

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Risks associated with silica nanoparticles

By the AVICENN team – Last added June 2022

General: increasingly documented adverse effects

Risks, classification and evaluation

Silica nanoparticles (SiO2) are used by industry in a wide range of applications, in the food industry, cosmetics, pharmaceuticals, diapers and sanitary napkins, but also elastomers, resins, paints and inks, etc.
As confirmed by the annual assessments of the French declaration of substances in the nanoparticulate state published since the end of 2013, various nanoforms of silica are imported or produced in France.

The potentially harmful effects of silica nanoparticles on healthSee1the references listed at the bottom of the page, section “elsewhere on the web” are increasingly documented.

In 2021, silica nanoparticles were identified as one of the four categories of nanoparticles most at risk by a team from University College Dublin2Cf. A semiquantitative risk ranking of potential human exposure to engineered nanoparticles (ENPs) in Europe, Li, Y and Cummins, E, Science of the Total Environment, 778, July 2021.

As early as 2014, the French Health Safety Agency (ANSES) had advocated a classification of silica dioxide nanoparticles in the CLP Regulation so that measures to restrict use or even ban the use of certain consumer applications could be put in place.

An assessment of silica dioxide and its nanoforms by the Netherlands was planned for 2012 under REACh, via the “Community Continuous Action Plan”(CoRAP) of the European Chemicals Agency (ECHA). It was launched in 2014Cf.3Community rolling action plan (CoRAP) update covering years 2014, 2015 and 2016, ECHA, March 2014 but was not successful due to lack of sufficient data from silica manufacturers.
In March 2015, ECHA requested more information from industry on the silica nanoforms they manufacture, in order to complete the evaluation of these substances4DECISION ON SUBSTANCE EVALUATION PURSUANT TO ARTICLE 46(1) OF REGULATION (EC) NO 1907/2006 For Silicon dioxide, CAS No 7631-86-9 (EC No 23 1-545-4), March 2015. But in June 2015, ECHA’s “Board of Appeal” was notified of appeals by nanosilica manufacturers refusing to give the requested information: one isolated, by Grace (Germany)5Announcement of appeal – Grace GmbH & Co. KG, ECHA, August 2015: “The Agency has based its decision very largely on its own classification of SAS as a nanomaterial, a classification that the Agency is not empowered to make and that in any event is irrelevant to the toxicity of SAS; (d) The Contested Decision is disproportionate in that it is not appropriate or necessary to achieve the objective of protecting human health, and places an unduly heavy burden on the Appellants”the other grouped, bringing together 35 companies6Announcement of appeal – Evonik Degussa GmbH and others, ECHA, August 2015: “On 29 February 2012, silicon dioxide was included on the CoRAP due to initial grounds for concern relating to ‘the substance characterisation, nanoparticles and toxicity of different forms of the substance’. The Appellants claim, however, that none of those alleged grounds for concern are criteria for inclusion of a substance on the CoRAP. The Appellants argue that as a result the Agencys decision to include the substance on the CoRAP was adopted in breach of Article 44 of the REACH Regulation and must be set aside. (…) The Appellants claim that the mere fact that the substance meets the non-legally binding definition of ‘nanomaterials’ in Commission Recommendation 2011/696/EU on the definition of nanomaterial is not sufficient to justify the requests for information in the Contested Decision. By requesting information on the substance on the grounds that the substance meets the non-legally binding definition of ‘nanomaterials’ in the Commission Recommendation, the Agency failed to identify a valid concern that needs to be addressed through the substance evaluation procedure.”
In addition to the French companies mentioned above, the signatories were companies :
– German: Evonik Degussa GmbH, Evonik Industries AG, Akzo Nobel Chemicals GmbH, BASF SE, Cabot Aerogel GmbH, Cabot GmbH, Clariant Produkte (Deutschland) GmbH, Grace Silica GmbH, Johnson Matthey Chemicals GmbH, Merck KGaA, Wacker Chemie AG
– Spanish: Evonik Silquilmica SA, IQESIL S.A, Instituto Suizo Para el Fomento de la Seguridad Swissi-España, S.L.U
– Belgium: Evonik Degussa Antwerpen NV, SCAS Europe S.A./N.V, Specialty Chemicals Coordination Center SA/NV
– Swedish: Akzo Nobel Pulp and Performance Chemicals AB
– Finland: Akzo Nobel Finland OY, Albemarle Europe Sprl, J.M. Huber Finland OY
– Dutch: Albermarle Catalysts Company B.V., PPG Industries Chemicals BV
– UK: Cabot Carbon Limited, LSR Associates Ltd, PQ Silicas UK Ltd, PPG CENTRAL (UK) Ltd.
– Italian: Deltagran Europe srl, Silysiamont SpA, Solvay Solutions Italia SpA
– Greek : Hellenic Petroleum SA
See also: 35 firms fight Echa decision on nano silicon dioxide, Chemical Watch, August 2015
Four companies based in France are among the signatories (Evonik Aerosil France Sarl, Clariant Production S.A.S, Merck Performance Materials SAS, Rhodia Operations SAS).
A March 2015 ECHA document states thata possible underestimation of hazards cannot be ruled out based on the data provided by silica manufacturers, who are called upon to clarify their information. What is the current status of this case? This is not very clear.

The results of a Silimmun research project conducted by French researchers and presented in 2021 show that amorphous silica nanoparticles, while not displaying the sustained pro-inflammatory character of crystalline silica, do exhibit marked effects on the cells of the immune system which could lead to deregulation of immune responses. However, the subject is far from being fully explored. In particular, genotoxicity should be analyzed in detail with respect to the internalization capacity of silica vs. the intrinsic defense of cells against genotoxic stress7See The effects of amorphous silica nanoparticles on the immune system, Thierry Rabilloud, The Research Papers. Health, Environment, WorkANSES, 2021, Microplastics and nanomaterials, pp.17-19, 2021.

Silica nanoparticles are likely to carry genotoxic agents on their surface which leads to aggravate their harmful effects on DNA8See Dussert F et al, Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents, Nanomaterials, MDPI, 10 (7): 1418, 2020.

Aggregates should not necessarily be considered less toxic than primary particles

In early 2020, the results of research conducted in Belgium were published showing that aggregates larger than 100 nm should not necessarily be considered less toxic than their nanoscale counterparts9Cf. Assessing the Toxicological Relevance of Nanomaterial Agglomerates and Aggregates Using Realistic Exposure In Vitro, Murugadoss S et al, Nanomaterials, 11, 1793, 2021 and Is aggregated synthetic amorphous silica toxicologically relevant, Murugadoss S et al, Particle and Fibre Toxicology, 17(1), 2020.

Specific risks of silica for different applications

Specific risks of silica in cosmetics

Regarding cosmetics, the Scientific Committee on Consumer Safety (SCCS) reported in September 2015 that too disparate, inadequate and insufficient data to be able to draw any conclusion concerning the safety of silica nanoforms10Opinion on Silica, Hydrated Silica, and Silica Surface Modified with Alkyl Silylates (nano form), Scientific Committee on Consumer Safety (SCCS), September 2015.
In 2019, the SCCS issued an opinion stating that none of the SAS materials (hydrophilic or hydrophobic) included in the dossier can be considered solubleCf11. Opinion on solubility of Synthetic Amorphous Silica (SAS), Scientific Committee on Consumer Safety (SCCS), June 20-21, 2019 (corrigendum December 6, 2019).

Specific risks of silica in food

Since at least 2011, it has been known that dietary silica (E551) contains nanoparticles, and researchers have estimated that we consume 124 mg of nano-silica per day.

Concerning the risks associated with their oral exposure (via food), the re-evaluation of silica in the form of E551 (nano and non-nano), was adopted by EFSA much later than the initial schedule, at the end of 2017, without definitive conclusions being drawn regarding the safety or toxicity of this additive12See in particular:
Re-evaluation of silicon dioxide (E 551) as a food additive, adopted November 2017, Younes M et al, EFSA Journal, 6(1):5088, 2018
Commission Regulation (EU) No 257/2010 of 25 March 2010 establishing a programme for the re-evaluation of approved food additives in accordance with Regulation (EC) No 1333/2008 of the European Parliament and of the Council on food additives
Food additives re-evaluation work programme, Paolo Colombo, Senior Scientific Officer – Food Additives Team, Food Ingredients and Packaging (FIP) Unit, EFSA, 28 April 2014
. A call for data was opened by EFSA between October 2018 and May 2020; in the absence of conclusive data, the current authorization of this food additive would be revised on the basis of EFSA’s current scientific opinion and the additive could be withdrawn from the European Union’s list of authorized additives. (There will be no new call for additional data).
While waiting for the results, scientists are reporting significant side effects.

The 1st nano substance authorized as a biocide

In April 2014, the synthetic amorphous silica nano dioxide became the first (and until the end of 2016 at least, the only) nano substance to have been approved for marketing as a biocidal substance as of November 1, 201513 COMMISSION EXECUTIVE REGULATION (EU) No 408/2014 approving synthetic amorphous silicon dioxide as an existing active substance for use in biocidal products of product-type 18, 23 April 2014.
See also our sheet What regulation of nanomaterials in biocides in Europe?
The antibacterial properties of silica, sought after for certain targeted applications, can have undesirable effects on certain bacterial communities necessary for the health or balance of ecosystems. Vigilance is therefore required.

And in the medical field?

In the medical field, a paper published on January 28, 2019, in Nature Nanotechnology shows that silica dioxide nanoparticles can induce changes in the endothelium and thus leakage of tumor cells, which causes metastasis. According to Frédéric Lagarce, professor of biopharmacy and hospital practitioner in Angers, “What is interesting / original is to show a potential risk of nanotechnologies in the treatment of tumors while these technologies are often presented as the answer to improve the performance of anticancer drugs. It is now necessary to verify if these endothelial modifications are also found with polymeric or lipidic nanoparticles, which are much more used to encapsulate active ingredients and target tumors. If this were unfortunately the case, the whole strategy of nanomedicine (very cancer-oriented) would be called into question.

Workers are the first to be exposed

In April 2019, the INRS had issued a call to companies using nanostructured amorphous silica for occupational health research. The candidates were obviously few, because the INRS has relaunched a new identical call in September 2021

In May 2019, the French National Health Security Agency (Anses) warned of the high health risks associated with crystalline silica, particularly for the 365,000 workers exposed to it, including quartz. The risks of amorphous silicas are particularly important, because of their nanometric size. The Anses recommends a series of measures in terms of prevention and control of exposure in the workplace, medical surveillance and recognition of occupational diseases.

In February 2022, the INRS published a specific safety sheet on synthetic amorphous silicas which outlines the prevention and protection measures to be implemented to protect workers.

Elsewhere on the web

In French :

Impact of the environment on the β-lactam-recognizing T-cell response: roles in allergic mechanisms, Alexia Feray. Toxicology. Université Paris-Saclay, 2021.
Synthetic amorphous silicas, Fiche pratique de sécurité, ED 153, INRS, February 2022
State of knowledge on the “toxicity of nanostructured amorphous silicas, Radauceanu A et al, Références en santé au travail n° 160, TP36, INRS, December 2019
Hazards, exposures and risks related to crystalline silica, Anses, April 2019 (published in May 2019)
Hazards, exposures and risks related to crystalline silica, Anses, April 2019 (published in May 2019)
– AVICENN, Nanomaterials and risks to health and the environment – Soyons Vigilants !, éditions Yves Michel, February 2016
– Francelyne Marano, Faut-il avoir peur des nanos ?, Buchet Chastel, April 2016
– “Internalization and translocation of silica oxide and titanium oxide nanoparticles in bronchial epithelial, pulmonary endothelial, and muscle cells” by Mornet S et al. in Participant’s file prepared for the Restitution of the National Environmental Health and Work Research Program (PNREST), October 2015
Evaluation of the risks associated with nanomaterials – Issues and knowledge update, ANSES, April 2014 (online May 15, 2014)
Risk assessment of nanomaterials for the general population and the environment (chapter 6.6: Food products and silica), Afsset (now ANSES), March 2010

In English:

Influence of Critical Parameters on Cytotoxicity Induced by Mesoporous Silica Nanoparticles, Ahmadi A et al, Nanomaterials,12(12), 2022
Silica nanoparticles induce cardiac injury and dysfunction via ROS/Ca2+/CaMKII signaling, Qi Y et al, Science of The Total Environment, 2022
Synthetic Amorphous Silica Nanoparticles Promote Human Dendritic Cell Maturation and CD4+ T-Lymphocyte Activation, Feret a et al, Toxicological Sciences, Oxford University Press (OUP), 185 (1): 105-116, 2022
Adverse effects of amorphous silica nanoparticles: Focus on human cardiovascular health, Guo C et al, Journal of Hazardous Materials, 406(15), 124626, 2021
The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies, Dong X et al, Int J Nanomedicine, 15: 9089-9113, November 2020
Silica nanoparticles induce endoplasmic reticulum stress response and activate mitogen activated kinase (MAPK) signalling, Toxicol Rep, Christen V and Fent K, 3:832-840, November 2016
Critical review of the safety assessment of nano-structured silica additives in food, Winkler HC et al, Journal of Nanobiotechnology, 14:44, 2016
Critical assessment of toxicological effects of ingested nanoparticles, McCracken C et al, Environ. Sci.: Nano, 3, 256-282, 2016
Oxidative stress, inflammation, and DNA damage in multiple organs of mice acutely exposed to amorphous silica nanoparticles, Nemmar A et al, Int J Nanomedicine, 11: 919-928, 2016
Biodistribution, excretion, and toxicity of mesoporous silica nanoparticles after oral administration depend on their shape, Li L et al, Nanomedicine: Nanotechnology, Biology and Medicine, 11(8): 1915-1924, November 2015
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route, Tarantini A et al, Environmental and Molecular Mutagenesis, 56 (2): 18-227, March 2015
Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure, part 2: Intratracheal instillation and intravenous injection, Guichard Y et al, Environmental and Molecular Mutagenesis, 56 (2): 228-244, March 2015
Toxicity, genotoxicity and proinflammatory effects of amorphous nanosilica in the human intestinal Caco-2 cell line, Tarantini A et al, Toxicology in Vitro, 29(2): 398-407, March 2015
Novel insights into the risk assessment of the nanomaterial synthetic amorphous silica, additive E551, in food, van Kesteren PCE et al, Nanotoxicology, 2014
Kinetics of silica nanoparticles in the human placenta, Poulsen MS et al, Nanotoxicology, July 2013but also on the environmentSee for example:
– Physiological and Behavioral Effects of SiO2 Nanoparticle Ingestion on Daphnia magna, Kim Y et al, Micromachines (Basel), 12(9): 1105, September 2021
Inhibition of total oxygen uptake by silica nanoparticles in activated sludge, Journal of Hazardous Materials, 283(11): 841-846, February 2015

A comment, a question? This sheet realized by AVICENN is intended to be completed and updated. Please feel free to contribute.

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